What Does the ‘Love Hormone’ Do? It’s Complicated

June 17, 2020

A new Weizmann Institute of Science study of mice in a semi-natural setting shows how the hormone oxytocin can amplify aggression as well as friendliness.

During the pandemic lockdown, as couples have been forced to spend days and weeks in one another’s company, some have found their love renewed while others are on their way to divorce court. Oxytocin, a peptide produced in the brain, is complicated in that way: a neuromodulator, it may bring hearts together or it can help induce aggression.

This is the conclusion from the unique research led by Weizmann Institute of Science researchers where mice living in semi-natural conditions had their oxytocin producing brain cells manipulated in a highly precise manner.

The findings, published in Neuron, could shed new light on efforts to use oxytocin to treat a variety of psychiatric conditions, from social anxiety and autism to schizophrenia.

Much of what we know about the actions of neuromodulators like oxytocin comes from behavioural studies of lab animals in standard lab conditions. These conditions are strictly controlled and artificial, in part so that researchers can limit the number of variables affecting behaviour. But a number of recent studies suggest that the actions of a mouse in a semi-natural environment can teach us much more about natural behaviour, especially when we mean to apply those findings to humans.

Professor Alon Chen’s lab group in the Weizmann Institute’s Neurobiology Department have created an experimental setup that enables them to observe mice in something approaching their natural living conditions – an environment enriched with stimuli they can explore – and their activity is monitored day and night with cameras and analysed computationally.

The study, which has been ongoing for the past eight years, was led by research students Sergey Anpilov and Noa Eren, and Staff Scientist Dr Yair Shemesh in Professor Chen’s lab group.

The innovation in this experiment, however, was to incorporate optogenetics – a method that enables researchers to turn specific neurons in the brain on or off using light. To create an optogenetic setup that would enable the team to study mice that were behaving naturally, the group developed a compact, lightweight, wireless device with which the scientists could activate nerve cells by remote control.

With the help of optogenetics expert Professor Ofer Yizhar of the same department, the group introduced a protein previously developed by Yizhar into the oxytocin-producing brain cells in the mice. When light from the wireless device touched those neurons, they became more sensitized to input from the other brain cells in their network.

“Our first goal was to reach that ‘sweet spot’ of experimental setups in which we track behaviour in a natural environment, without relinquishing the ability to ask pointed scientific questions about brain functions,” said Anpilov.

Shemesh added: “The classical experimental setup is not only lacking in stimuli, the measurements tend to span mere minutes, while we had the capacity to track social dynamics in a group over the course of days.”

Delving into the role of oxytocin was a test drive for the experimental system. It had been believed that this hormone mediates pro-social behaviour. But findings have been conflicting, and some have proposed another hypothesis, termed ‘social salience’ stating that oxytocin might be involved in amplifying the perception of diverse social cues, which could then result in pro-social or antagonistic behaviours, depending on such factors as individual character and their environment.

To test the social salience hypothesis, the team used mice in which they could gently activate the oxytocin-producing cells in the hypothalamus, placing them first in the enriched, semi-natural lab environments. To compare, they repeated the experiment with mice placed in the standard, sterile lab setups.

In the semi-natural environment, the mice at first displayed heightened interest in one another, but this was soon accompanied by a rise in aggressive behaviour. In contrast, increasing oxytocin production in the mice in classical lab conditions resulted in reduced aggression.

“In an all-male, natural social setting, we would expect to see belligerent behaviour as they compete for territory or food,” said Anpilov.

“That is, the social conditions are conducive to competition and aggression. In the standard lab setup, a different social situation leads to a different effect for the oxytocin.”

If the ‘love hormone’ is more likely a ‘social hormone’ what does that mean for its pharmaceutical applications?

“Oxytocin is involved, as previous experiments have shown, in such social behaviours as making eye contact or feelings of closeness,” said Eren.

“However our work shows it does not improve sociability across the board. Its effects depend on both context and personality.”

This implies that if oxytocin is to be used therapeutically, a much more nuanced view is needed in research:

“If we want to understand the complexities of behaviour, we need to study behaviour in a complex environment. Only then can we begin to translate our findings to human behaviour,” she said.

Participating in this research were scientists at the Max Planck Institute for Psychiatry in Munich, including research students Asaf Benjamin and Stoyo Karamihalev, staff scientist Dr Julien Dine and postdoctoral fellow Dr Oren Forkosh of the Chen lab; Professor Shlomo Wagner and postdoctoral fellow Dr Hala Harony-Nicolas of Haifa University; Professor Inga Neumann and research student Vinicius Oliveira of Regensburg University, Germany; and electrical engineer Avi Dagan.

More Research

Social Hierarchy: Even for Mice, It’s Complicated

New research from the Weizmann Insitute of Science shows that by studying wild mice in near-natural conditions, different strategies used by females and males in forming social hierarchies were revealed….

eastRead More

A Hundred Million Suns: The Most Complete Portrait of a Supernova

Humankind has long turned to the skies in search of answers. Accounts of supernovae – exploding stars – go back thousands of years, but while we know today that these…

eastRead More

Fungus vs. Fungus: Newly Identified Yeast Might Prevent Life-Threatening Fungal Infections

Researchers at the Weizmann Institute of Science have identified a yeast that might be used to prevent invasive candidiasis, a major cause of death in hospitalised and immunocompromised patients. The…

eastRead More

Toward a Treatment for Huntington’s Disease

Weizmann Institute of Science scientists have discovered two small molecules that can cross the blood-brain barrier in mice, slowing and even reversing the effects of Huntington’s, which is incurable. The…

eastRead More

View All